Novo Nordisk 

Compound Sharing

Novo Nordisk 

Compound Sharing


InsR peptide antagonist (S961)

Amount per vial
1000 nmol

Insulin binds to the insulin receptor (InsR), which exists in two alternatively spliced isoforms, InsR-A and InsR-B. The insulin receptor belongs to the same family of receptor tyrosine kinases as the IGF1 receptor (IGF1R) and insulin is therefore also able to bind to the IGF1R albeit with considerably lower affinity as compared to the InsR.

NNC0069-0961 (also known as S961) is a single chain peptide consisting of 43 amino acids, which has been shown to be a specific InsR antagonist. The affinity of S961 for the InsR is comparable to that of human insulin and the selectivity for the insulin receptor versus the IGF1 receptor is higher than that of human insulin. S961 is also an antagonist of the InsR of other species such as pig and rat and it also has considerable affinity for hybrid receptors consisting of InsR and IGF1R. S961 completely inhibits insulin action, both in cellular assays and in vivo in rats.

Thus, S961 exhibit high affinity and selectivity for the InsR and can be used to unravel the role of InsR signaling and function in both in vivo and in vitro experiments.

Selected calculated properties for NNC0069-0961 are listed in the table.
MW (Da)4802.1
pI (calculated)3.99
Extinction coefficient (calculated),280 nm19605

2D sketch of the structure of NNC0069-0961. The compound is composed of natural amino acids and contains one disulfide bridge.

Receptor binding affinities for NNC0069-0961 is given in the table (based on the Schäffer et al. 2008 reference listed in the reference section).

The binding affinity of NNC0069-0961 for various InsR variants are given in the table below. The affinities were determined by competition assays either in a scintillation proximity assay (SPA) or a PEG precipitation assay format using solubilized holoreceptors (see the Schäffer et al. 2008 reference listed in the reference section for further details on the in vitro assays).

(IC50, pM)
Human insulin 
(IC50, pM)
Human InsR-A affinity (SPA assay, solubilized receptor), no HSA48 29
Human InsR-B affinity (SPA assay, solubilized receptor), no HSA
Rat InsR-A (PEG assay, solubilized receptor), no HSA5627
Pig InsR-A (PEG assay, solubilized receptor), no HSA

HSA: human serum albumin; InsR-A: insulin receptor isoform A, InsR-B: insulin receptor isoform B; PEG: polyethylene glycole; SPA: scintillation proximity assay

S961 induces hyperglycemia, hyperinsulinemia and insulin resistance in rats. S961 treatment induces significant increases in the plasma glucose and insulin levels. S961 induces glucose intolerance as determined by a glucose tolerance test. S961 treatment led to impaired insulin-mediated glucose disposal as determined by an insulin tolerance test. 

Please see the Vikram and Jena 2010 reference listed in the reference section for further details.

Human insulin (NNC0121-0308) is available as a reference compound to NNC0069-0961. Please indicate in the 'Purpose' field when you order NNC0069-0961 if you would like to have human insulin (NNC0121-0308) included in your shipment.

Peptides and proteins have a tendency to adhere to glass and plastic surfaces. This may at low concentration impact the actual amount in solution. To minimize this unspecific adherence, adding detergents or inert proteins like e.g., ovalbumin or other serum albumins to the solution can minimize this phenomenon. In case albumins are added to peptide/protein solutions, ensure that the albumins are free of any proteases, Recommended procedure for in vitro studies: dissolve the entire content of the vial by adding 4 mL 30 mM HEPES buffer pH 8. Gently rotate the vial until all content is dissolved. Avoid harsh shaking or stirring of the solution. Keep the stock solution at 4C overnight and make the desired number of aliquots (use low protein binding vials) of the stocks. Snap freeze the aliquots in liquid nitrogen and store them at minus 20C. When thawed, the stock solution should be stable for up to three weeks at 4C. Recommended procedure for in vivo studies: NNC0069-0961 can be dosed in vivo in a formulation vehicle containing 10mM sodium phosphate, 140mM sodium chloride, (0.007% polysorbate 20 if concentrations are so low that adsorption to vials may affect the concentration), pH 7.4. Formulations should be used fresh, but can be stored for up to one week refrigerated.

Schäffer L et al.
A novel high-affinity peptide antagonist to the insulin receptor
Biochem Biophys Res Commun. 2008 Nov 14;376(2):380-3
Vikram A, Jena G
S961, an insulin receptor antagonist causes hyperinsulinemia, insulin-resistance and depletion of energy stores in rats
Biochem Biophys Res Commun. 2010 Jul 23;398(2):260-5