Novo Nordisk 

Compound Sharing

Novo Nordisk 

Compound Sharing


Long-acting GLP-1 analogue #1

Amount per vial
1000 nmol

NNC0090-1170 (also known as liraglutide) is a GLP-1 analogue with 97% sequence homology to human GLP-1 that binds to and activates the GLP-1 receptor. Compared to native GLP-1, NNC0090-1170 have been designed to be long-acting in vivo. The main mechanism for the extended half-life is albumin binding. Furthermore, the NNC0090-1170 has a delayed uptake from the subcutis.

GLP-1 action is mediated via a specific interaction with GLP-1 receptors, leading to an increase in cyclic adenosine monophosphate (cAMP). GLP-1 stimulates insulin secretion in a glucose-dependent manner. Simultaneously, GLP-1 lowers inappropriately high glucagon secretion, also in a glucose­dependent manner. Thus, when blood glucose is high, insulin secretion is stimulated and glucagon secretion is inhibited. Conversely, during hypoglycaemia GLP-1 diminishes insulin secretion and does not impair glucagon secretion. GLP-1 is a physiological regulator of appetite and food intake and the GLP-1 receptor is widely expressed in the brain.

Selected calculated properties for NNC0090-1170 and GLP-1 (7-37]-OH are listed in the table.
PropertyNNC0090-1170 GLP-1 (7-37)-OH
MW (Da)
pI (calculated)
Sequence substitutions (compared to reference)
26K(C16-gGlu), 34R

Extinction coefficient (calculated, 280 nm)

2D sketch of NNC0090-1170. NNC0090-1170 has a C16 fatty acid chain which is attached via a gamma glutamic acid linker to lysine at position 26. In addition, lysine is replaced with arginine at position 34. The fatty acid side chain enables reversible binding to serum albumin in the blood stream, which increases the half-life of the molecule.
3D model of NNC0090-1170 based on a crystal structure and modelling of the fatty acid side chain.
2D sketch of human GLP-1 (7-37)-OH (NNC0113-0007).

All in vitro data are based on in vitro assays using cloned human GLP-1 receptors expressed in baby hamster kidney cells. Since albumin binding is a key mechanism for the design of NNC0090-1170, the apparent affinity and potency will be very dependent on whether the assays contain albumin or not. See the Lau et al. 2015 reference listed in the reference section for further details on the experimental setup of the in vitro assays that have been used to generate the data in the table.

hGLP-1R binding (IC50, nM +/- SEM)
0% HSA
2% HSA
GLP-1 (7-37)-OH0.19 +/- 0.030.10 +/- 0.02
NNC0090-11700.11 +/- 0.024.78 +/- 1.01

hGLP-1R potency (EC50 pM, +/- SEM)
GLP-1 (7-37)-OH16.2 +/- 0.9
NNC0090-11708.5 +/- 0.7
hGLP-1R: human GLP-1 receptor; HSA: human serum albumin

The terminal half-life of NNC0090-1170 is around 14 h in pigs while shorter in mice, rats, rabbits and monkeys (4-8 h).

NNC0090-1170 has been evaluated in several diabetic and obese animal models. NNC0090-1170 shows a dose-dependent and long-lasting anti-hyperglycaemic effect in ob/ob and db/db mice. The mean area under the curve (AUC) for blood glucose is dose-dependently reduced after a single subcutaneous injection of NNC0090-1170 (30, 100, 300 or 1000 ug/kg). In db/db mice, the ED50 for lowering of blood glucose (6 hours post dosing) is estimated to be approximately 24 ug/kg following an acute dose. With twice daily administration of 100 ug/kg NNC0090-1170 for two weeks, blood glucose AUCs were significantly reduced in ob/ob mice. These anti-hyperglyeamic effects are also observed in db/db mice treated for 15 days with NNC0090-1170 (200 ug/kg twice daily s.c.).

In young ZDF rats, 6 week treatment with NNC0090-1170 (30 and 150 ug/kg twice daily s.c.) reduces blood glucose levels and insulin levels following a glucose tolerance test. In candy-fed rats, NNC0090-1170 (0.2 mg/kg, twice daily, s.c.) reduces food intake and subsequently weight gain, especially fat mass. 

When administering NNC0090-1170 to animals it is necessary to do dose titrations. A good dose for pharmacological experiments could be 1 mg/kg once daily titrated up from 0.1 mg/kg to 0.3 mg/kg to 1.0 mg/kg.

Human GLP-1(7-37)-OH (NNC0113-0007) is available as a reference compound to NNC0090-1170. Please indicate in the 'Purpose' field when you request NNC0090-1170 if you would like to have NNC0113-0007 included in your shipment.

Peptides and proteins have a tendency to adhere to glass and plastic surfaces. This may at low concentration impact the actual amount in solution. To minimize this unspecific adherence, adding detergents or inert proteins like e.g., ovalbumin or other serum albumins to the solution can minimize this phenomenon. In case albumins are added to peptide/protein solutions, ensure that the albumins are free of any proteases, but be aware that it will affect the apparent potency and affinity in in vitro assays in case a fatty acid is attached to the compound. For in vitro studies, NNC0090-1170 can be dissolved in 80/20% DMSO/MilliQ water (e.g. at a concentration of 300 uM). NNC0090-1170 can be dosed in vivo in a formulation vehicle containing 50mM sodium phosphate, 70mM sodium chloride, (0.007% polysorbate 20 if concentrations are so low that adsorption to vials may affect the concentration), pH 8.0. Formulations should be used fresh, but can be stored for up to one week refrigerated.

Knudsen LB et al.
Potent derivatives of glucagon-like peptide-1 with pharmacokinetic properties suitable for once daily administration
J Med Chem. 2000;43(9):1664-9
Madsen K et al.
Structure-activity and protraction relationship of long-acting glucagon-like peptide-1 derivatives: Importance of fatty acid length, polarity, and bulkiness
J Med Chem. 2007;50(24):6126-32
Lau J et al.
Discovery of the once-weekly glucagon-like peptide-1 (GLP-1) analogue semaglutide
J Med Chem. 2015;58(18):7370-80
Knudsen LB, Lau J
The Discovery and Development of Liraglutide and Semaglutide
Front Endocrinol. 2019;10:155
Knudsen, LB
Inventing Liraglutide, a Glucagon-Like Peptide‑1 Analogue, for the Treatment of Diabetes and Obesity
ACS Pharmacol. Transl. Sci., 2019, 2, 468-484