NPY receptor scintillation proximity assays (SPA) were performed
using cell membrane preparations from cell lines expressing one of the
human Y1, Y2, Y4, or Y5 receptors. Reduction of cyclic adenosine
monophosphate (cAMP) through Gi-coupled Y receptor activation was
measured using human embryonic kidney (HEK) 293 cells stably
expressing one of the human Y1, Y2, Y4, or Y5 receptors and a
cAMP-sensitive calcium channel. See the Østergaard et al.
2018 reference listed in the reference list for further data and
details on the experimental setup of the in vitro assays.
|Receptor binding data
|Potency data (EC50, nM)|
Y1, Y2, Y4, Y5 are NPY receptor subtype 1,2,4, and 5, respectively